Collagel Hydrogel (Rat)
Catalog No: HG017
Size: 20 mL
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| Product Description | Hydrogel |
| Application | 3-D Cell Cultures |
| Purity | > 95% |
| Biological Source | Rat |
| Storage/Stability | Keep frozen at -20 °C. |
| Shelf Life | ≥12 months |
| Note | Thaw your Collagel Hydrogel (Rat) sample bottle at room temp or in a 37 °C water bath, invert product while thawing. When you see a small amount of ice left in your sample, transfer the bottle into an ice bath. It is important to keep CollaGel Hydrogel on ice since it will solidify at temperatures above 8 °C. ✓ Place a sterile magnetic stir bar in a sterile beaker. ✓ Place plate containing ice on a stir plate. ✓ Place the sterile beaker containing the stir bar on the ice bath. ✓ Pour the Collagel Hydrogel (Rat) into the beaker carefully, try to avoid air bubbles. ✓ Slowly start stirring Collagel Hydrogel (Rat) solution. ✓ Add your wanted media to the Collagel Hydrogel (Rat) solution. Judge the pH visually by the phenol red in the media then add your cell suspension. ✓ Pipette your wanted volume of the mixture into each well. ✓ Let 6-well plate sit at room temp for 10-15 minute before placing in the incubator. ✓ 1 Hour later add your wanted volume of media on top of each Collagel Hydrogel (Rat) (2-2.5 mL). ✓ Use within 1 week, change media every 2 days or as needed. |
! For Research/Industry Use Only!