Human Gelatin Methacrylate
Catalog No: PS024
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| Product Description | Gelatin is a heterogenous mixture of water-soluble proteins with high average molecular masses. Gelatin proteins are derived by denaturing collagen-rich tissue in water. The mechanical properties of gelatin, such as gel stiffness and compressibility, are related to the average molecular weight of the gelatin proteins and characterized by its Bloom number. Human Gelatin Methacrylate is a methacrylated version of Human Gelatin. |
| Application | Due to the excellent biocompatibility of gelatin, it has been used to generate 3D scaffolds and hydrogels for tissue engineering applications, and as a delivery material to control the release of bioactive molecules. In the pharmaceutical industry, gelatin is used as an encapsulating agent and binder for tablet production.Its ability to be photocrosslinked allows for a tunable 3D hydrogel with a wide range of applications including 3D bioprinting, 3D cell culturing, and many other tissue engineering and regenerative medicine research applications. |
| State | Lyophilized |
| Biological Source | Human |
| Storage/Stability | Stored in airtight containers at ambient temperature (15-30 °C) upon receipt and prior to solubilization. |
| Shelf Life | 6 months after opening |
| Note | Human Gelatin Methacrylate can be reconstituted in 37°C water or PBS to the desired concentration with agitation/vortexing for several minutes. Dissolving time depends on the gelatin concentration but usually takes a few minutes for low concentration. Centrifugation might be used to remove undissolved particulate. After reconstitution, the solution should be used immediately or stored at 2-10°C. Solubilized gelatin can be sterilized by filtration through a 0.22 µm membrane. Human Gelatin Methacrylate - Crosslinking Note: The following are general recommendations. Researchers should optimize parameters based on their specific application. Dissolve Human Gelatin Methacrylate in 60°C 1x PBS or 1x cell culture media at the desired concentration. The recommended final concentration is ≥ 5%. a. Mechanical properties of the hydrogel can be controlled by varying the Human Gelatin Methacrylate concentration, the photoinitiator concentration, and the degree of cross-linking. Dissolve the photoinitiator and keep the solution protected from light. a. Irgacure 2959 can be dissolved in neat methanol or DMSO at a 10% concentration. b. LAP can be dissolved in PBS at a 2.5% concentration. Add the desired amount of photoinitiator to the Human Gelatin Methacrylate solution. a. Depending on the application and bioprinting parameters, a photoinitiator concentration between 0.1 – 0.5% is recommended. In a sterile environment, use a syringe filter to sterile filter the Human Gelatin Methacrylate. Place sterile Human Gelatin Methacrylate in a 37°C incubator. Add the cells to the sterile Human Gelatin Methacrylate/photoinitiator solution. Load the cell/Human Gelatin Methacrylate/photoinitiator solution into the bioprinting syringe specific to the bioprinter according to the manufacturer’s instructions. Set up the bioprinter and optimize parameters based on the printing condition, intended application, and bioprinter manufacturer instructions. The parameters needing to be optimized include nozzle diameter, pressure, nozzle temperature, print bed temperature, print speed, and crosslinking light source distance and intensity. a. These are important parameters to optimize to achieve the desired extrudability of the Human Gelatin Methacrylate from the nozzle. Good extrudability means the bioink flows out of the nozzle as a filament as opposed to droplets. b. Based on the photoinitiator used, crosslinking settings will vary. i. For LAP, the crosslinking light should be at a wavelength of 405 nm. ii. For Irgacure 2959, the crosslinking light should be at a wavelength of 365 nm. |
! For Research/Industry Use Only!