Dextran (SG)-based HA-Link Hydrogel Kit
Catalog No: HG006
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| Product Description | The Dextran (SG)-based HA-Link Hydrogel Kit provides reagents for setting up hydrogels containing hyaluronic acid. Its major components are SG-Dextran and the thiol-functionalized hyaluronic acid crosslinker HA-Link. When SG-Dextran and HA-Link are combined, thiol groups on HA-Link form stable thioether bonds with thiol-reactive groups on SG-Dextran, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation. The hydrogel allows cell spreading and migration if cell adhesion peptides are present in the gel. Prior to the crosslinking step, cell adhesion peptides can be covalently attached to a portion of the SH-reactive groups on SG-Dextran to provide a cell-adhesive matrix. Dextran hydrogels can be dissolved by the addition of dextranase, which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation. |
| Application | 3-D cell culture, hydrogel injections, filling of microchannels, generation of soft to very stiff gels. |
| Components | SG-Dextran: 170 µL, 30 mmol/L HA-Link, lyophilized: 2 x 200 µL*, 10 mmol/L* 10x CB (pH 7.2): 200 µL Water: 2 x 1500 µL |
| Note | Allows formation of 3.3 mL Dextran(SG)-based HA-Link Hydrogel of a crosslinking strength of 1.2 mmol/L. |
! For Research/Industry Use Only!