PVA (SG)-based CD-Link Hydrogel Kit (slow)
Catalog No: HG025
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| Product Description | The PVA (SG)-based CD-Link Hydrogel Kit provides reagents for setting up slow gelling, cell-compatible hydrogels. Its major components are polymeric SG-PVA and the crosslinker CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with thiol-reactive groups on SG-PVA, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation. CD-Link is composed of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala). The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9. It allows cells to spread and migrate within the hydrogel if they express the indicated MMPs. In most cases cell spreading and migration also requires the presence of adhesion peptides. Prior to the cross-linking step, peptides can be covalently attached to a portion of the SH-reactive groups of SG-PVA to provide a cell-adhesive matrix. |
| Application | 3-D cell culture |
| Components | SG-PVA, lyophilized: 170 µL*, 30 mmol/L* CD-Link, lyophilized: 200 µL*, 20 mmol/L* 10x CB (pH 5.5): 200 µL Water: 2 x 1500 µL |
| Note | Allows formation of up to 2 mL PVA(SG)-based CD-Link Hydrogel Kit depending on the grade of stiffness of the gel. |
! For Research/Industry Use Only!