ECM Gel from Engelbreth-Holm-Swarm murine sarcoma
Catalog No: HG007
Size: 1 mL,5 mL,10 mL
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| Product Description | ECM was prepared to a protein concentration of 8-12 mg/mL (in DMEM), containing laminin as a major component, collagen type IV, heparin sulfate proteoglycan, entactin, and other minor components. Addition of collagen type IV to the ECM gel increases polymerization. |
| Application | ECM gel can used with epithelial cells, endothelial cells, muscle cells, nerve cells and tumor cells. |
| State | Liquid |
| Concentration | 8-12 mg/mL |
| Components | ECM was prepared to a protein concentration of 8-12 mg/mL (in DMEM), containing laminin as a major component, collagen type IV, heparin sulfate proteoglycan, entactin, and other minor components. Addition of collagen type IV to the ECM gel increases polymerization. Product contains 50 mg/L Gentamicin Sulfate. |
| Biological Source | Mice |
| Storage/Stability | For long term storage, keep product at -20 °C. The ECM gel may be stored at 2-8 °C for up to 72 hours. |
| Note | This product is prepared from Engelbreth-Holm-Swarm sarcoma in mice; dialyzed against chloroform. Gel should be thawed overnight at 2-8°C before use and dispensed to a multiwall plate using a plate and pipettes that are pre-cooled to 2-8°C. The gel may also be diluted up to twofold with 2-8°C Dulbecco′s Modified Eagle′s Medium, and should be done before gel is added to the plate. The product will gel within 5 minutes at 20°C. For prolonged manipulations, work should be conducted below 10°C. Cells can be plated on top of a thin gel layer of 0.5 mm or cultured inside a 1 mm layer. If you culture cells inside, add the cells to the gel prior to plating at a recommended density of 3-4 x 104 cells per mL. To dissociate cells from this gel, use protease in PBS without calcium, magnesium, or EDTA, at a concentration of 0.6-2.4 units per mL. |
! For Research/Industry Use Only!