Dextran (Mal)-based CD-Link Hydrogel Kit (Fast)
Catalog No: HG002
Online Inquiry
| Product Description | The Dextran (Mal)-based CD-Link Hydrogel Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized dextran (Mal-Dextran) and the crosslinker CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with maleimide groups on the Mal-Dextran, which results in the formation of the gel. CD-Link is composed of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala). The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9. It allows cells to spread and migrate within the hydrogel if they express the indicated MMPs. In most cases cell spreading and migration also requires the presence of adhesion peptides. Prior to the crosslinking step, cell adhesion peptides can be covalently attached to a portion of the maleimide groups of Mal-Dextran to provide a cell-adhesive matrix. Dextran hydrogels can be dissolved by the addition of dextranase, which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation. |
| Application | 3-D cell cultures |
| Components | Mal-Dextran, lyophilized: 170 µL*, 30 mmol/L* CD-Link, lyophilized: 200 µL*, 20 mmol/L* 10x CB (pH 5.5): 200 µL Water: 2 x 1500 µL |
| Note | Allows formation of up to 2 mL Dextran(Mal)-based CD-Link Hydrogel depending on the stiffness of the gel. |
! For Research/Industry Use Only!